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このアイテムの引用には次の識別子を使用してください: http://hdl.handle.net/10126/4823

タイトル: Cytoskeletal and Ultrastructural Studies on Regenerating Cells from the Protoplasts of Valonia (Siphonocladales, Chlorophyceae)
著者: Paul Rommel, Elvira
Kazuo, Okuda
キーワード: actin filament
cortical microtubule
protoplast
Valonia
雑誌名: 黒潮圏科学
発行日: 26-Mar-2011
出版者: 高知大学大学院総合人間自然科学研究科黒潮圏総合科学専攻黒潮圏科学編集委員会
抄録: Ultrastructure and cytoskeletal properties of the coenocytic green alga, Valonia, were described using light field, immunofluorescence and electron microscopy to investigate the dynamics among cell wall, cell membrane, and protoplasm during cell regeneration. Protoplasts were artificially induced in three species by cutting thalli and extruding the protoplasm. Protoplasts contracted and formed irregularly shaped masses within 30 minutes concomitant with bundling of actin filaments (AFs), convolution of cortical microtubules (CMTs) and formation of a thin enveloping membrane composed of polysaccharides. Size affected survival rates: protoplasts less than 10 μm in diameter displayed lower viability than larger protoplasts. A new cell wall was produced within 24 hours simultaneous with CMT and AF depolymerization. AFs were reduced to granular structures and aggregates that repolymerized by 48 hours. Concurrently, new CMTs polymerized and attained a parallel arrangement. Actin- and microtubule-destabilizing agents had variable effects on protoplast contraction indicating a minor role of intact cytoskeletons in this process; however, resulting cells exhibited abnormal protoplasm distribution and cell deformation after three days. Rhizoids began to form after 7 days on untreated cells which subsequently produced lateral branch cells that eventually developed into mature thalli.
URI: http://hdl.handle.net/10126/4823
出現コレクション:01 黒潮圏科学=Kuroshio Science   1(2007)-

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