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01 Bulletin of marine sciences and fisheries, Kochi University (高知大学海洋生物研究報告)  12(1992)-25(2007) >

このアイテムの引用には次の識別子を使用してください: http://hdl.handle.net/10126/4366

タイトル: ブリ連鎖球菌症に対する経口ワクチンのマイクロカプセル化
その他のタイトル: Encapsulation of oral delivery vaccine against Lactococcus garvieae infection in yellowtail Seriola quinqueradiata
著者: 川合, 研児
畑本, 琴美
Kawai, Kenji
Hatamoto, Kotomi
キーワード: Microcapsule
oral vaccine
Lactococcus garvieae
雑誌名: Bulletin of Marine Sciences and Fisheries, Kochi University
発行日: 1-Mar-2000
出版者: 高知大学海洋生物教育研究センター
抄録: Oral vaccine against streptococcus infection in yellowtail Seriola quinqueradiata is already commercialized, however the vaccine has disadvantageous property that much amount is necessary to induce enough efficacy. One of the proposed reasons for it is that antigenicity of the administrated antigen is decreased while it passes the stomach of fish. Present study is conducted to develop encapsulated vaccine to protect antigen from the effect of stomach. Firstly, culture and inactivation conditions of the bacterium Lactococcus garvieae were examined to get highly antigenic cells. L. garvieae S−2433 strain was cultured with and without shaking in brain heart infusion broth. Antigenicity was determined by agglutination using antisera against two types of L. garvieae. Antigenicity was also determined after inactivation with various concentrations of formalin and by heating at different temperatures. Encapsulation of vaccine was carried out by the method of mixing acetone dissolution of cellulose acetate phthalate to antigen dispersed in paraffin oil. Confirmation of encapsulation of the antigen was carried out using Ouchterlony immunodiffusion test. The highest agglutination of the bacterium was found when it was cultured 9-12 h with shaking at 30℃. High antigenicity of the bacterium is shown when it was inactivated with 0.2 % formalin or by heating for 30 min at 100℃. The diameter of the capsule preparation ranged 30-70 μm, showing spherical body. The result of Ouchterlony test showed that the antigen was encapsulated and the encapsulated antigen was released at neutral pH but not at low pH. Thus, a possibility that this enteric-coated microcapsule is available for the development of new oral vaccine.
URI: http://hdl.handle.net/10126/4366
出現コレクション:01 Bulletin of marine sciences and fisheries, Kochi University (高知大学海洋生物研究報告)  12(1992)-25(2007)


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