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01 Bulletin of marine sciences and fisheries, Kochi University (高知大学海洋生物研究報告)  12(1992)-25(2007) >

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タイトル: Seed production and cultivation of Grateloupia turuturu (Cryptonemiales, Rhodophyta) by callus culture
著者: Huang, Wei
Fujita, Yuji
Ninomiya, Masayori
Ohno, Masao
キーワード: callus
Grateloupia turuturu
red alga
seed production
tissue culture
雑誌名: Bulletin of Marine Sciences and Fisheries, Kochi University
発行日: 1-Mar-2000
出版者: 高知大学海洋生物教育研究センター
抄録: Application of tissue culture techniques to seed production and cultivation of the red alga Grateloupia turuturu Yamada was examined. Callus masses excised from explants of G. turuturu exhibited an undifferentied growth on solid ASP12NTA (1.2% agar) medium supplemented with 0.1 mg/L of indole-3-acetic acid (IAA) and 6-benzylaminopurine (BAP). Growth of the callus was maintained for over five years by subculture every 2-3 months. The callus cells were able to attach to twines of Nori-net and oyster shell, following which they developed into crusts in PES liquid medium. The seeds were observed from upright development of the crusts. The young thalli grew to 3-5 mm in length after 6-8 weeks in room culture. For cultivation in sea, the Nori-nets were suspended from experimental rafts at Nagasaki and Kochi coast. The thalli grew most rapidly from January to April. After five months growth, the thalli attained a length of approxirnately which is similar to naturally growing thalli.
URI: http://hdl.handle.net/10126/4358
出現コレクション:01 Bulletin of marine sciences and fisheries, Kochi University (高知大学海洋生物研究報告)  12(1992)-25(2007)


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