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01 Bulletin of marine sciences and fisheries, Kochi University (高知大学海洋生物研究報告)  12(1992)-25(2007) >

このアイテムの引用には次の識別子を使用してください: http://hdl.handle.net/10126/4351

タイトル: Production of Monoclonal Antibodies against Yellowtail Leucocyte
著者: Ishikawa, Takahide
Kawai, Kenji
キーワード: Monoclonal antibody
classification of leucocytes
雑誌名: Bulletin of Marine Sciences and Fisheries, Kochi University
発行日: 31-Dec-1998
出版者: 高知大学海洋生物教育研究センター
抄録: BALB/c mouse was immunized with yellowtail peripheral blood leucocytes (PBLs) and spleen cells removed from the immunized mouse were fused with myeloma by using polyethyleneglycol method. Antibody producing hybridomas were screened by ELISA using mixed leucocytes as target antigen. A total of 8 hybridomas (culture no. AB-2, AB-10, BB-11, BC-5, BC-6, CF-11, DG-10 and EG-11) were obtained by single cell cloning. Monoclonal antibodies (MAbs) produced in the culture supernatant of cloned hybridomas were used for further characterization. Imunofluorescent stain using FITC labeled rabbit secondary antibody revealed that MAbs AB-2, AB-10 and EG-11 recognized lymphocyte and MAbs BC-5, CF-11 and DG-10 recognized granulocyte or monocyte. MAbs BC-6 and BB-11 reacted to yellowtail lymphocyte which bore immunoglobulin of yellowtail.
URI: http://hdl.handle.net/10126/4351
出現コレクション:01 Bulletin of marine sciences and fisheries, Kochi University (高知大学海洋生物研究報告)  12(1992)-25(2007)


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