Kochi University Digital Repository for Academic Resources Kochi University

Kochi University Repository >
92  総合研究センター Science Research Center >
01 Bulletin of marine sciences and fisheries, Kochi University (高知大学海洋生物研究報告)  12(1992)-25(2007) >

このアイテムの引用には次の識別子を使用してください: http://hdl.handle.net/10126/4346

タイトル: Practical Manual on Detection of DNA Polymorphism in Fish Population Study
著者: Nugroho, Estu
Takagi, Motohiro
Taniguchi, Nobuhiko
キーワード: DNA polymorphism
practical manual
fish population genetics
雑誌名: Bulletin of Marine Sciences and Fisheries, Kochi University
発行日: 31-Dec-1997
出版者: 高知大学海洋生物教育研究センター
抄録: In order to support an activity of conservation of valuable natural population and biological diversity in aquatic environment, the valuable population genetic data are necessary. In our laboratory, Department of Aquaculture-Kochi University, beside allozymes which have been established for more twenty years ago for revealing those genetic data, some molecular genetic techniques such as Random Amplified Polymorphic DNA (RAPD); Multilocus DNA Fingerprinting; Amplified Restriction Fragment Polymorphism (AFLP); and Variable Number Tandem Repeats (VNTR) of Microsatellite, have also been used. During study of the DNA polymorphism in several fish species, we conclude that the RAPD and multi-locus DNA fingerprinting (including AFLP) analysis are most useful for pedigree study or species identification, while population genetic parameters will be more accurately estimated by single locus of microsatellite DNA. This paper shows a protocol for detection of fish DNA polymorphism in our laboratory.
URI: http://hdl.handle.net/10126/4346
出現コレクション:01 Bulletin of marine sciences and fisheries, Kochi University (高知大学海洋生物研究報告)  12(1992)-25(2007)


ファイル 記述 サイズファーマット
marine17-109.pdf1.68 MBAdobe PDF見る/開く



高知大学学術情報基盤図書館 - ご意見をお寄せください